Table 1. Measures of behavioral and histological tests in the normal group and the control group.
Test | Parameter | Norm | Cont | p # |
Balance beam test | Number of paw slipping | 0.15±0.10 | 0.17±0.16 | 0.933 |
Completion time (sec) | 18.67±4.67 | 19.45±7.21 | 0.869 | |
Grip strength test | Hang time (sec) | 11.66±3.51 | 11.47±4.43 | 0.904 |
Water maze test | Latency (sec) | 37.09±6.30 | 41.42±10.77 | 0.558 |
Number of target site crossovers | 10.80±2.78 | 11.20±1.93 | 0.723 | |
Nissl staining | Cell count (/mm2) | 3224.00±365.04 | 3175.00±207.76 | 0.772 |
NeuN labeling | Cell count (/mm2) | 2458.60±96.80 | 2471.20±119.13 | 0.917 |
Darpp32 labeling | Cell count (/mm2) | 2007.20±56.85 | 1941.80±102.22 | 0.188 |
Interneuron number | Parv+ (/mm2) | 79.48±1.79 | 78.86±2.72 | 0.783 |
Cr+ (/mm2) | 65.76±2.35 | 65.84±1.92 | 0.959 | |
NPY+ (/mm2) | 49.20±1.48 | 48.90±1.95 | 0.828 | |
ChAT+ (/mm2) | 69.34±2.59 | 69.60±1.55 | 0.899 | |
Fiber density | Parv+ (/100 μm) | 13.04±0.97 | 13.02±0.96 | 0.978 |
Cr+ (/100 μm) | 6.97±0.34 | 7.04±0.56 | 0.943 | |
NPY+ (/100 μm) | 5.98±0.40 | 6.03±0.75 | 0.926 | |
ChAT+ (/100 μm) | 3.06±0.24 | 3.16±0.40 | 0.777 | |
Varicosity density | Parv+ (/100 μm) | 4.36±0.71 | 4.04±0.79 | 0.599 |
Cr+ (/100 μm) | 6.98±1.05 | 7.18±1.63 | 0.875 | |
NPY+ (/100 μm) | 6.22±0.79 | 5.80±1.10 | 0.540 | |
Western blots | NeuN (OD) | 1.29±0.08 | 1.31±0.04 | 0.493 |
Darpp32 (OD) | 1.28±0.07 | 1.28±0.05 | 0.822 | |
Parv+ (OD) | 1.19±0.10 | 1.21±0.06 | 0.634 | |
Cr+ (OD) | 0.67±0.08 | 0.68±0.05 | 0.731 | |
NPY+ (OD) | 1.13±0.10 | 1.13±0.11 | 0.971 | |
ChAT+ (OD) | 0.48±0.05 | 0.49±0.02 | 0.733 |
Note: values expressed as group means±SD; # normal (Norm) v.s. control (Cont), one-way ANOVA and Fisher's post hoc PLSD test.