Skip to main content
. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Anal Biochem. 2013 Dec 9;448:41–49. doi: 10.1016/j.ab.2013.12.001

Figure 5.

Figure 5

Loading exosomes with SPION5. B16 melanoma exosomes were loaded with SPION5 passively or by electroporation in the presence of trehalose pulse media at 0.75 kV/cm. (a) B16 melanoma exosomes visualized by TEM in the absence of electroporation or post electroporation with SPION5; the black lower left corner scale bar = 100 nm (b) Selected stages of SPION5 loading or unloading of exosome contents are observed by TEM after electroporation. (c) SPION5 loaded exosome size was assessed with DLS (n = 6; error bars = s.d.) (d) DiI labeled SPION5 loaded exosomes were isolated on a representative continuous sucrose gradient, fractions corresponding to peak exosome densities collected, densities determined and exosomes detected using Xenogen IVIS. (e) SPION5 loaded exosome iron content was assessed for exosome peak fractions using ICP-OES (n =3; error bars = s.d.). EP = electroporated; F = fraction