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Comparison of UVR-induced aggregation of HγD-Crys constructs with aromatic pairs replaced by alanines. Samples were prepared at 1 mg/mL in reaction buffer, and light scattering was monitored at 600 nm during the UVR exposure time. (a) N-terminal pairs: WT (black diamonds), Y6A/F11A (red triangles), Y16A/Y28A (green hashes), and Y45A/Y50A (blue circles). (b) C-terminal pairs: WT (black diamonds), Y92A/Y97A (orange squares), F115A/F117A (lavender dashes), Y133A/Y138A (turquoise X’s).
