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. 2014 Mar 14;9(3):e91346. doi: 10.1371/journal.pone.0091346

Figure 2. S1PR1 is responsible for barrier integrity in S1P stimulated HUVECs.

Figure 2

(A) HUVECs were transfected with a control siRNA SMARTpool (siCTRL) or a siRNA SMARTpool targeting S1PR1 (siS1PR1) or S1PR3 (siS1PR3) and grown to confluence on electrode ECIS-arrays. (B) SiCTRL, siS1PR1 or siS1PR3 transfected HUVECs show no differences in impedance after the indicated time points. (C) HUVECs transfected with a control siRNA SMARTpool (siCTRL) or a siRNA SMARTpool targeting S1PR1 (siS1PR1) or S1PR3 (siS1PR3) were incubated with 1 μM S1P or SF medium alone. The impedance was continuously monitored and was corrected for starting impedance after 1 hr serum starvation. Three independent experiments were performed. Statistical significance was assessed by 2-way ANOVA followed by Bonferroni post-hoc test for selected comparison (***P<0.001; **P<0.01 and *P<0.05).