Table 1. Primer sequences used in Real-time PCR verification.
| Gene symbol | Associated Porcine RefSeq Accession No. | Primer | Primer sequence (5'-3') | Product size (bp) |
| LGMN | XM_001927082 | Forward | AGACGCTCCACAAACAGTAC | 95 |
| Reverse | CAACTTCATGGCAGAGATGGA | |||
| GATA2 | NM_213879 | Forward | CTCCAGCTTCACCCCTAAG | 157 |
| Reverse | CCCGTTCATCTTGTGGTACAG | |||
| KRT8 | NM_001159615 | Forward | AGATCCAAAAGCGTACCGAC | 136 |
| Reverse | AGCTGCCTGTAGAAGTTGATC | |||
| PSEN2 | NM_001078666 | Forward | CTCAACTCCGTGCTCAACA | 148 |
| Reverse | GATGTAGGTGAAGAGGAAGAGC | |||
| NCSTN | XM_001928786 | Forward | CCCCGCAATGTCATGTTTG | 92 |
| Reverse | AACTTGCCCTTCTCCATATCG | |||
| HES1 | NM_001195231 | Forward | CTGGAGAAGGCGGACATTC | 92 |
| Reverse | GCTCGGGTCTGTGCTTAG | |||
| HEY2 | NM_001243329 | Forward | CTGCAAAGTTAGAAAAGGCCG | 145 |
| Reverse | TCTGTTAAGCACTCTCGGAATC | |||
| ANXA8 | NM_001243599 | Forward | AGACATACAAGCAGATACCAGTG | 142 |
| Reverse | CTTCTCACCCGCTGCATAC | |||
| SLC36A2 | XM_003134141 | Forward | CATCACCCAGTACATCATCCAG | 127 |
| Reverse | CAGAACCACACCAATGCTTTC | |||
| KCTD3 | XM_003357619 | Forward | AGAAGTTCCCTCTGCGAATG | 149 |
| Reverse | CGTACCATAGGCGATCTCAATC | |||
| NANOG | NM_001129971 | Forward | GGACTTTTCCTACAATCCAGC | 153 |
| Reverse | CCCATAAACCTCAGGCATTG | |||
| JAG1 | XM_001926559 | Forward | ACATAGCCCGAAACAGTAGC | 158 |
| Reverse | GTTGTAGCAGGGATGAGGAC | |||
| DPP4 | NM_214257 | Forward | TGCGGATTCCATACCCAAAG | 137 |
| Reverse | ATCCCCTATTAACACAGACGC | |||
| KRT18 | XM_003126180 | Forward | TTGACCGTGGAGTTGGATG | 149 |
| Reverse | ACCACTGAGGTGCTCTCC | |||
| Xeno | Control primer Xeno from SYBR Green Cells-to-CT Control Kit (Ambion) | 105 | ||