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. 2014 Mar 14;9(3):e91745. doi: 10.1371/journal.pone.0091745

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© 2014 Damania et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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HBx protein was over-expressed in both Huh 7 and Hep G2 cells (A). HBx was over-expressed in hepatoma cells and the empty vector was used as transfection control. Lane 1, Control; lane 2, Empty vector; and lane 3, HBx transfected cells. Cell proliferation assay was performed using WST1 reagent in Huh 7 (B) and Hep G2 (C) cells. After 48 hours of transfection the proliferation assay was performed. (n = 3; *p<0.01).