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. 2014 Mar 14;9(3):e91745. doi: 10.1371/journal.pone.0091745

Figure 4. Role of miRNA-21 on proliferation of hepatoma cells.

Figure 4

miRNA-21 was over-expressed in Hep G2 (A & C) and Huh 7 (B & D) cells. Relative expression of miRNA-21 was measured in the transfected cells (A & B) to determine the miRNA-21 levels (n = 3; *p<0.05 and **p<0.01). As an internal control, 5S RNA was quantified in all real time PCR experiments. Both C & D show the cell proliferation assays in miRNA-21 over-expressing cells. Cells were plated at a density of 8000/well in a 96-well plate for the proliferation assays. As a control in all these experiments NS-miRNA was used in parallel experiments (n = 3; *p<0.05).