Representative MS/MS analysis of DMG-DAG species in the neutral loss and precursor-ion scan modes. Neutral loss scan of DMG (103 Da) (NLS103) from an equimolar mixture of DMG-DAG species at 1 pmol/µl each in the presence of 0.1% formic acid was acquired at collision energy of 27 eV (A), indicating protonated DMG-DAG species. NLS103 (B) and PIS110 (C) of the equimolar DMG-DAG mixture in the presence of LiOH acquired at collision energy of 39 and 76 eV, and collision gas pressure of 1.0 and 0.6 mTorr, respectively, displayed lithiated DMG-DAG species. NLS87 (D) of the equimolar DMG-DAG mixture in the presence of LiOH acquired at collision energy of 39 eV identified 1,3-DMG-DAG species.