Table 1. List of primer with their sequence and the method where they have been used.
Primer name | Sequence (5′ -> 3′) | Method |
R2-F | AGCGCCCACAGGATTTYCGNCCNAT | C |
R2-R | AGGGCGAACATCCGCTGYTGBGGT | C |
EploUp70-F | TGCCCAAGTGCTCTGAATTGTC | C, TP |
EploDown70-R | AGATAGGGACAGTGGGAATC | C |
EploR2_2800-R | CGAGATGGTAGAGCACTAATC | TP, CN, E |
EploR2_2500-F | CAAGTCCCTCCATGCTCGCCA | TP, CN, E |
Eplo R2_820-R | GGTGCCCGCTTAATGATGTCC | C, TP |
Eplo R2_720-R | GGAGTGGGCCATCGCCAGATC | TP |
Eplo R2_1200-R | GTAATCGGTGCCCACAGATC | TP |
EploR2_670-F | AAGGTCGACAACAGTGTCATC | C |
Eplo R2_600-R | AATAGAGTCCAACGTCCGGTC | TP |
ITSFISH4 | ATATGCTTAAATTCAGCGGG | CN |
ITS3 | GCATCGATGAAGAACGCAGC | CN |
C: Initial cloning of element; CN: Copy number estimation; E: Expression analysis; TP: 5′ Truncation profile.