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. 2014 Mar 14;9(3):e91848. doi: 10.1371/journal.pone.0091848

Figure 8. Drp-1 siRNA prevents Mn damage.

Figure 8

Western blot analysis of Drp-1 protein in C6 cells transfected with pooled Drp-1 or control siRNA for 48 hours. Reprobing with an anti-β-Actin antibody was performed to normalize for protein loading. Results are expressed as a percentage of the respective control considered as 100%. Viability was measured by MTT assay (A); Quantification of mitochondrial morphology and Δψm dissipation analysis using MitoTracker Red CMXRos (75 nM) (B); Apoptotic nuclei were determined by Hoechst 33258 staining (arrowheads). Scale bar: 10 µm (C). Results are average of three individual experiments. Statistical significance, **p<0.01 and ***p<0.001 vs. control; ###p<0.001 vs. Mn.