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. 2014 Mar 14;9(3):e91953. doi: 10.1371/journal.pone.0091953

Figure 1. PDZ is necessary to accomplish an efficient nuclear export of L-PRX.

Figure 1

(A) Structures of L-PRX and its PDZ-deleted mutants (DPDZ-L-PRX). Amino acid residues flanking each domain are numbered on top of the diagram. (B) Subcellular localization of EGFP-tagged L-PRX and its PDZ-deleted mutant (DPDZ-L-PRX). RSC96 cells were transfected with the plasmids encoding EGFP-tagged full-length L-PRX and its DPDZ-L-PRX. Scale bar = 25 μm. (C) GFP fusion constructs detected by Western blotting to ensure that the visualized proteins are not free GFP, or GFP fused to a truncated protein.