Figure 5. Nuclear accumulation of L-periaxin by mutating Leu₈₃ and Leu₈₅ to Gln.

(A) Structures of L-PRX, PDZ-cyclin A1, and their mutants. Gray boxes indicate the amino acid residues replaced with Gln in the PDZ domain. (B) RSC96 cells were transfected with the plasmids encoding EGFP-tagged L-PRX, PDZ-cyclin A1, and their mutants. Scale bar = 25 μm. (C) The cytoplasm and nucleus were separated and determined the distribution of protein in the two fractions by Western blotting. β-actin and Histone served as an internal control for cytosol and nucleus, respectively. (D) Quantitative analysis of the distribution of EGFP fusion proteins in the two fractions by normalized to the internal control level. *P<0.05.