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. 2014 Mar 14;9(3):e92013. doi: 10.1371/journal.pone.0092013

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© 2014 Bertuccio et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Co-IP of HA-tagged KCa3.1 and either WT or DN Rab1 (Flag) was carried out in HEK293 cells as described in the Methods. Total cell lysates were subject to IP using either an α-HA Ab (lanes 5, 6) or an α-V5 Ab as IgG control (lanes 3, 4) and subsequently blotted for Rab1 (α-Flag). B. Co-IP of HA-tagged KCa3.1 and either endogenous (bottom panel) or GFP-tagged WT and DN Rab8 (top panel). Total cell lysates were subject to IP as in A and subsequently IB for either endogenous Rab8 (α-Rab8) or GFP-tagged Rab8 (α-GFP). Lysates for WT and DN Rab1 or 8 are shown in the first two lanes of each blot (5 μg loaded per lane). These data confirm an association between KCa3.1 and Rabs1 and 8. Data are representative of 3 experiments.