Figure 1. Toll-like receptors gene expression in human corneal epithelial (HCE) cells stimulated with A. castellanii trophozoites or LPS. HCE cells were treated with A. castellanii trophozoites (1×105 cells/ml) or LPS (10 μg/ml) for 24 hours following which cells were processed for total RNA isolation and RT-PCR.
The amount of mRNA expression was quantified by densitometry of bands in comparison to the Glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Densitometry of mRNA bands were quantified by three independent scanned presented as mean±SEM.