Figure 5. Effect of TLR4 neutralizing antibody on IL-8 production.
HCE cells incubated with or without A. castellanii (1×105 cells/ml) or LPS (10 μg/ml) for 24 hours. Inhibition of TLR4 activity was carry out by pre-incubating HCE cells for 1 hour with neutralizing TLR4 antibody (10 μg/ml) of anti-hTLR4 affinity purified goat IgG, or with the control antibody (10 μg/ml) normal human IgG, followed by incubation with or without A. castellanii (1×105 cells/ml) or LPS (10 μg/ml) for 24 hours. HCE cells incubated without treatment, served as control untreated group. Supernatants were collected and IL-8 secretion was quantified by ELISA. Data are mean±SEM expressed in % fold change of IL-8 production of three independent experiments. Asterisk indicates P value < 0.05 by unpaired Student’s t-test.