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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Mol Microbiol. 2014 Jan 7;91(3):618–634. doi: 10.1111/mmi.12482

Fig. 6. Tp0750-mediated MMP substrate degradation.

Fig. 6

A fluorescence-based degradation assay was used to determine if recombinant Tp0750 is capable of cleaving a MMP substrate. A negative control protein (Tp0327; 400 ng) and Tp0750 (400 ng) +/− the serine protease inhibitor AEBSF were incubated with either the MMP substrate (MMP; 2.5 μg) or the negative control substrate (ACE; 2.5 μg) (Tp0750 only) for 0–64 h. MMP substrate cleavage was measured by detecting the increase in relative fluorescence units (RFU) using standard fluorescein excitation/emission filters (320 nm/420 nm). Average fluorescence intensity readings from triplicate measurements are presented with bars indicating standard error (SE) and the results are representative of three independent experiments.