Table 2.
Primers used in RT-PCR and recombinant protein expression.
| ORF1 | Primer Orientation | Sequence2 |
|---|---|---|
| Tp0750 (RT-PCR) | Sense | 5′-TCAAAGTACCGTGGCAAACA |
| Tp0751 (RT-PCR) | Antisense | 5′-CCTCGTGGGATTCAAATGTT |
| Tp0750_G23-A199 (pET28a) | Sense | 5′-CTAGACCATATGGGTGAACGCACTGTCC |
| Tp0750_G23-A199 (pET28a) | Antisense | 5′-GTCAGCTCGAGTCAAGCTGCGATGCTGCGATAC |
| Tp0750_G23-A199 (pET32a) | Sense | 5′-CTACTACCATGGCTGGTGAACGCACTGTCC |
| Tp0750_G23-A199 (pET32a) | Antisense | 5′-CTACTACTCGAGTTAAGCTGCGATGCTGCGATAC |
| Tp0750_G23-D223 (pDEST17) | Sense | 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTGCGAACGCACTGTCCCCG |
| Tp0750_G23-D223 (pDEST17) | Antisense | 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTTCTAGTCAAGCGAAGGGG |
| Tp0750_G23-D223 (pAcGP67B) | Sense | 5′-CTACTACCATGGCTGGTGAACGCACTGTCCCC |
| Tp0750_G23-D223 (pAcGP67B) | Antisense | 5′-CTACTAGCGGCCGCGTCAAGCGAAGGGGTATAGCG |
| Tp0327_I23-S172 (pET28a) | Sense | 5′-CATGACCATATGATCACGCGCTTTGCCGTC |
| Tp0327_I23-S172 (pET28a) | Antisense | 5′-GTCAGCTCGAGTCACGAGCTGCTCAGCTC |
1
Shown in brackets is either the methodology that the primers were used for [RT-PCR] or the expression vector used for recombinant expression of each protein.
2
Restriction sites are highlighted in bold.