Extended Data Figure 7. GM-CSF treatment and L-Myc deficiency in bone marrow pDCs, splenic CD8α⁺ DCs and CD8α⁻ DCs.

a, Shown is a Venn diagram of probe sets changed at least 2-fold in expression between the indicated WT and L-Myc-deficient DC populations on gene expression microarray analysis. Indicated in each zone of the diagram is the number of probe sets. b, Shown are gene symbols and Gene Ontology (GO) biological process annotations for selected growth-related genes obtained from the analysis in (a). c, CD8α⁺ DCs (CD11c⁺MHCII⁺CD24⁺SIRPα⁻B220⁻) and CD8α⁻ DCs (CD11c⁺MHCII⁺SIRPα⁺CD24⁻B220⁻) were purified by cell sorting from the spleen of WT mice and cultured with media alone (Untreated) or with GM-CSF as indicated. After 24 h, cells were analyzed for viability. Shown are two-color histograms of 7AAD and AnnexinV expression. Numbers indicate the percent of cells in each quadrant gate. Also shown are single-color histograms of forward scatter (FSC-A) for live cells (AnnexinV⁻7AAD⁻) to determine relative size for the indicated treatments. Data are representative of 3 independent experiments. d, Shown are Wright-Giemsa stains of cytospins prepared from CD8α⁺ DCs described in (c). Scale bars represent 20 μm. e, Gene expression microarray analysis was performed using CD8α⁺ DCs described in (c). Shown is an M-plot of log₂-transformed normalized expression values for probe sets either increased (red) or decreased (blue) at least 2-fold in expression on treatment with GM-CSF, omitting probe sets lacking gene annotations. f, Functional annotations for 500 probe sets most induced by GM-CSF treatment (e, red) were clustered using the highest classification stringency by DAVID Bioinformatics Resources. Shown are the associated GO term, enrichment score, and number of contributing genes for the top 12-enriched clusters.