Figure 2. Mycl1 is restricted to dendritic cells in lymphoid and peripheral tissues and regulated by IRF8 and GM-CSF.

a, CD11c and MHCII expression is shown for GFP⁺ cells from spleen, inguinal lymph node (ILN), and mesenteric lymph node (MLN) from Mycl1^+/gfp mice (n=5 mice). b, CD11b and GFP expression in CD45⁺CD11c⁺MHCII⁺ cells from indicated tissues of WT and Mycl1^+/gfp mice (n=5mice). c, Immunofluorescence for indicated markers from Mycl1^+/gfp mice. Scale bars, 200 μM. d, Western analysis for L-MYC and PLC-γ2 from Flt3L-derived DCs from WT, Mycl1^gfp/gfp, and Csf2rb^−/− mice, treated as indicated for 24 h. e, IRF8 binding in the Mycl1 locus determined by ChIP-Seq in WT or Batf3^−/− DCs. Numbers represent normalized reads. Data representative of at least 3 experiments.