Figure 4. Mycl1 supports normal T cell priming by DCs following infection but mediates resistance to lethal Listeria monocytogenes challenge.

a-b, CD8⁺ T cells from wild type (WT) and Mycl1^gfp/gfp (KO) mice infected with LM-OVA treated with SIINFEKL were analyzed for TNF-α and IFN-γ production (a). Data (a-b) are from 2 independent experiments (bar, s.d., n=12). c, CD45.1 OT-I T cells transferred into the indicated mice and infected with LM-OVA were measured after 7 days. Numbers are OT-I T cells as a percent of all splenocytes. d, Total OT-I CD8⁺ T cells were measured from the indicated recipient mice after infection as described in (c). (bar, s.d., n=8). e, Survival of WT and Mycl^gfp/gfp (KO) mice after infection with L. monocytogenes. (bar, s.d., n=15). f, L. monocytogenes was measured in purified CD8α⁺ DCs after 2 h or 24 h of infection from (e) as described⁵². g, Splenic CD8α⁺ DCs from mice infected for 2 h were cultured in vitro in media with the indicated antibiotic for 12 h and viable intracellular bacteria quantitated as described⁵². (bar, s.d., n=4). h, Viable intracellular bacteria was measured as in (g) from the indicated cells from WT and Mycl1^gfp/gfp (KO) mice infected with L. monocytogenes for 60 h. (bar, s.d., n=3). **, p<0.001, ns, p>0.05.