Figure 1.

(A) Polymerase chain reaction (PCR) gels for Cre-recombinase (Top panel) and Shp2^flox/flox gene from tail snip samples with analysis indicating presence of Cre-recombinase and Shp2^flox/flox. Positive (+) indicates presence of Cre (loxP sequence). The WT is C57 negative control for Cre whereas “+ positive” is a positive Cre control sample that is added to the gel. The presence of floxed Shp2 gene product, the + sign indicates homozygote and + positive” is a positive floxed control sample that is added to the gel and negative (-) control” is just negative (no bands) control samples containing or not the specific gene product of the floxed Shp2 gene. The WT sample represents a sample from a regular C57 mouse containing only the normal gene product of the Shp2 gene product, and (B) Bottom panel, western blot data showing the ratio between Shp2 protein expression by beta-actin from hypothalamus, forebrain cortex and hindbrain of Shp2^flox/flox and Shp2/CamkIIα-cre mice. * p<0.05 compared to Shp2^flox/flox mice (n=5)