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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Acta Neuropathol. 2013 Nov 5;127(4):539–551. doi: 10.1007/s00401-013-1204-8

Figure 1. NMO-IgG binds to AQP4 in rat astrocytes and produces CDC in the presence of rat complement.

Figure 1

a. (Top left) AQP4 and GFAP immunofluorescence in well-differentiated primary cultures of rat astrocytes. (Bottom) Binding of NMO-IgG (recombinant human antibody rAb-53) or control IgG purified from NMO patient serum (each stained green with anti-human secondary antibody) to AQP4 (red). b. CDC (by Alamar blue assay) in rat astrocyte cultures following 3-h incubation with 5% rat complement and NMO-IgG or IgG purified from NMO patient serum (S.E., n=6). c. (Left) Immumofluorescence of brain sections at 3 h after intracerebral injection of 10 μg NMO-IgG showing area of antibody diffusion (white line). (Right) Expanded micrograph of boxed region along with data for control IgG.