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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Mol Microbiol. 2013 Dec 12;91(3):438–451. doi: 10.1111/mmi.12470

Fig. 3. Cytosolic catalase activity, mitochondrial superoxide dismutase activity and glutathione levels are decreased in ncr1Δ mutant cells.

Fig. 3

S. cerevisiae BY4741 and ncr1Δ::KanMX4 cells were grown in SC-glucose medium to PDS phase.

A,B. The activity of superoxide dismutase (SOD) and catalase was measured spectrophotometrically. Values are mean ± SD of at least three independent experiments. **p<0.01, unpaired Student’s t-test.

C,D. The activity of superoxide dismutases (Sod1p and Sod2p) or cytosolic catalase (Ctt1p) was assessed in situ after native-PAGE. One representative experiment out of three is shown.

E. Total glutathione levels. Reduced (GSH) + oxidized (GSSG) glutathione was measured as described in methods. Values are mean ± SD of at least three independent experiments. **p<0.01, unpaired Student’s t-test.