Fig. 5. Levels of long-chain sphingoid bases.
S. cerevisiae BY4741, ncr1Δ::URA3, sch9Δ and ncr1Δsch9Δ cells were grown in SC-glucose medium to the exponential (log) and post-diauxic shift (PDS) phase. Levels of indicated long chain bases were measured by HPLC-MS/MS. A. PHS - phytosphingosine; B. PHS-1-P – phytosphingosine-1-phosphate; C. DHS– dihydrosphingosine; D. DHS-1-P – dihydrosphingosine-1-phosphate. Data are expressed as pmol of lipid per total cell number (1.9×109) and are mean ± SD of three independent experiments. ****p<0.0001, ***p<0.001, **p<0.01, *p<0.05; Two-way ANOVA and Bonferroni test.