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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Mol Microbiol. 2013 Dec 12;91(3):438–451. doi: 10.1111/mmi.12470

Fig. 6. Ncr1p deficient cells exhibit increased levels of Sch9p and Sch9p-phospho-T570 and ncr1Δ phenotypes are suppressed by disruption of PKH1 or SCH9 but not by myriocin.

Fig. 6

S. cerevisiae BY4741, ncr1Δ::URA3, pkh1Δ, ncr1Δpkh1Δ, sch9Δ and ncr1Δsch9Δ cells were grown in SC-glucose medium to exponential (A and B) or PDS (C and D) phase.

A. Immunoblot analysis of Sch9p and P-T570-Sch9p. Pgk1p was used as loading control. . A representative experiment out of three is shown.

B,C. Hydrogen peroxide resistance (B) and chronological lifespan (C) were measured as in Fig. 1A and 2A, respectively. Values are mean ± SD of at least three independent experiments. ****p<0.0001, ***p<0.001 (relative to ncr1Δ); Two-way ANOVA and Bonferroni test.

D. Analysis of chronological lifespan in S. cerevisiae BY4741 and ncr1Δ::KanMX4 cells treated with ethanol (vehicle) or myriocin (600 ng mL−1; Myr). Values are mean ± SD of at least two independent experiments.