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. Author manuscript; available in PMC: 2015 Mar 5.
Published in final edited form as: Neuron. 2014 Mar 5;81(5):1111–1125. doi: 10.1016/j.neuron.2014.01.012

Figure 4. mAChRs Modulate Glutamate Release and Enhance DSE.

Figure 4

(A) 1 μM Oxo-M depresses fEPSP amplitude, which is blocked in the presence of atropine. (B) Oxo-M-induced eEPSC depression is blocked by pirenzepine and 4-DAMP pretreatment. (C) Oxo-M increases PPR, which is blocked by pirenzepine and 4-DAMP pretreatment. (D) Representative traces of Oxo-M-induced eEPSC depression under vehicle, pirenzepine and 4-DAMP conditions. (E) Distribution of M1 receptor (red) and the dendritic marker MAP2 (green) in the CeAL at low magnification; higher magnification shows punctate M1 staining in close apposition to MAP2 positive dendritic shafts (arrows in inset) (E1; scale bar 100μm, E2; bar 5μm, inset 7.5μm). (F) 1μM Oxo-M enhances DSE induced by 10 second depolarization. (G) PPR is increased by 10 second depolarization in both control and Oxo-M conditions. (H) Representative traces of control and Oxo-M DSE. (I) DSE in the presence of OXO-M is attenuated by THL and in CB1−/− mice. (J–K) Effects of pirenzepine and 4-DAMP on DSE in the presence of 1μM Oxo-M; grey faded lines represent Oxo-M only DSE condition from (I) for visual comparison purposes. (L) Summary data of the effects of THL, CB1 deletion, pirenzepine, and 4-DAMP on 10 second DSE in the presence of Oxo-M. **p<0.01, ***p<0.001, ****p<0.0001. Scale bars: 10ms, 100pA. Data presented as mean ± SEM. Also see Figure S2.