Skip to main content
. Author manuscript; available in PMC: 2014 Mar 15.
Published in final edited form as: J Allergy Clin Immunol. 2011 Feb 12;127(4):1054–62.e1-2. doi: 10.1016/j.jaci.2010.12.015

FIG 3. Allele-specific protein-DNA interactions at PHF11 confirming the ability of only Oct-1 to bind at rs1046295.

FIG 3

A, Competition EMSAs using Daudi nuclear extract. Rs1046295 allele A (lanes 1-8) and G (lanes 9-16). Molar excesses of unlabeled competitor oligonucleotides for the Oct-1 and SRY consensus sequences, and an unrelated probe (Unrel.), are indicated. B, EMSAs performed for rs1046295 allele A using Daudi and CCL-159 nuclear extracts and Oct-1 (POU2F1) and SRY recombinant proteins. Addition of antibodies for SRY or YY1 (negative control) is indicated (lanes 7-9).