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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Curr Alzheimer Res. 2013 Jun;10(5):532–541. doi: 10.2174/1567205011310050009

Fig. (6). BRI2 reduces the mRNA levels of BACE1.

Fig. (6)

HEK293 cells transfected with the cDNA encoding for BACE1 and the myc-tag bearing vector pRK5 (lane 1) or the cDNA encoding for mycBRI2 (lane 2). Part of the cells was used to prepare cell extracts and part to prepare mRNA and cDNA. (A) Cell extracts were analyzed by western blot using the anti-myc antibody 9E10 (panel a) or anti-BACE1 antibody 7523 (panel b). (B) The cDNA was used in Real-Time PCR reactions in order to calculate the absolute concentration of the mRNA of BACE1 and GAPDH and their ratios, which were plotted in the barogram. The experiment was repeated three times and the values are the average. The error bars represent the standard deviation (SD), + S.D. 0,074, p < 0,01.