Figure 1.

Process Flow in Multiplex Cytokine Analysis. Using a suspension phase array system (Luminex) cytokines are captured onto the surface of fluorescently coded microspheres. A biotinylated detector antibody is then bound in a sandwich configuration to the captured cytokine, washed and tagged. The amount of captured cytokine is then determined by measuring the amount of streptavidin conjugated flourescent probe complexed on the bead relative to a standard curve of pure cytokine