Fig 3. IL-2 contributes to the enhanced proliferation and survival of anti-CD3-activated Id1^tg CD4⁺ T cells.

(A) IL-2 levels in culture supernatants 2 days after anti-CD3 stimulation was measured by ELISA in triplicates. A representative of three experiments is shown as mean±SEM. (B) Cell proliferation assay of WT and TG naïve CD4⁺ T cells stimulated with anti-CD3 (1 µg/ml) for 3 days with or without IL-2 (2 ng/ml) or anti-IL-2 neutralizing antibodies (10µg/ml). (C) Cell survival was analyzed by PI/Annexin V staining. Cells were stimulated with anti-CD3 (1 µg/ml) for 3 days with or without IL-2 (5 ng/ml) or anti-IL-2 antibodies. Data shown are representatives of 4 experiments. *** p<0.001 indicates comparison between WT and TG cells. # p<0.05, ## p<0.01 and ### p<0.001 show comparison between with or with anti-IL-2 treatment.