Fig 4. Enhanced NF-κB activation in Id1^tg CD4⁺ T cells upon anti-CD3 stimulation.

Sorted WT and TG naïve CD4⁺ naïve T cells were treated with anti-CD3 (2 µg/ml) with or without anti-CD28 (2 µg/ml) for 18 hours and then harvest for nuclear extract. An aliquot was reserved for no treatment control. EMSA was done with ³²P-labeled specific NF-κB probe as previously described [18]. NF-κB binding complexes and a non-specific band (ns), which serves as an internal control, are as marked.