Table 1.

Method validation of kisspeptin-10 in rat plasma.

	Concentration (ng/mL)
	0.5	1	5	50	500
Within-run #
1	0.45	1.21	5.58	50.37	628.88
2	0.50	1.36	6.21	54.94	556.13
3	0.51	1.10	5.84	50.65	574.99
4	0.45	1.30	6.08	53.37	566.30
5	0.55	0.89	5.97	51.19	588.15
6	0.49	1.03	5.10	47.20	516.16
Average ± SD	0.49 ± 0.04	1.15 ± 0.18	5.80 ± 0.40	51.29 ± 2.67	571.77 ± 37.17
CV(%)	7.8	15.4	7.0	5.2	6.5
Accuracy	98.3	115.0	116.0	102.6	114.4
Recovery (%)	76.0	83.9	103.3	97.8	81.5
Between-run #
1	0.47	0.99	5.23	50.30	510.24
2	0.65	1.00	4.77	50.25	523.12
3	0.58	0.95	5.04	50.03	512.78
4	0.50	1.00	5.58	50.83	507.02
5	0.46	1.11	5.05	50.18	523.12
6	0.49	1.27	5.16	46.26	512.05
Average ± SD	0.53 ± 0.07	1.05 ± 0.12	5.14 ± 0.27	49.64 ± 1.68	514.72 ± 6.80
CV(%)	14.2	11.3	5.2	3.4	1.3
Accuracy	105.0	105.3	102.8	99.3	102.9