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Construction of recombinant vectors pET-28a-forw-pepc2 and pET-28a-reve–pepc2. The Chlamydomonas reinhardtii pepc2 (Crpepc2) fragment was ligated into pMD18-T to construct the intermediate vector pMD18-T-Crpepc2. The forward vector pMD18-T-Crpepc2 was digested with BamHI, and the fragment was inserted into plasmid pET-28a to generate pET-28a-pepc2. Digestion with XbaI distinguished between forward vector pET-28a-forw-pepc2 and reverse vector pET-28a-reve-pepc2
