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a Cloning of the pepc2 gene fragment. M 500 bp marker; lane 1, pepc2 gene fragment. b Identification of the forward vector pMD-18-T-Crpepc2 by BamHI digestion. M 500 bp marker; lane 1, pMD18-T-Crpepc2+digested with BamHI. c Identification of forward vector pET-28a-forw-pepc2 and reverse vector pET-28a-reve-pepc2 by XbaI digestion. Lane 1, pET-28a-reve-pepc2 digested with XbaI; lane 2, pET-28a-forw-pepc2 digested with XbaI; M 500 bp marker
