Table 1.

Relative expression levels of pepc in wild type, control, forward and reverse mutants

Strains	Cta (gapdh b)	Cta (pepc)	Relative expressionc (%)
Wild type	27.03 ± 0.14	32.26 ± 0.18	100
Control mutant	27.30 ± 0.01	32.71 ± 0.37	88
Forward mutant	27.32 ± 0.02	30.70 ± 0.13	362
Reverse mutant	27.19 ± 0.04	34.23 ± 0.22	29

Samples were measured at 6 h after IPTG induction via RT-qPCR

Values presented are the mean of three replicates

^aCt was obtained from the amplification curves qPCR experiments

^bGlyceraldehyde-3-phosphate dehydrogenase (gapdh) was used as a reference gene

^cRelative expression was calculated as 2^−∆∆Ct ∆∆Ct = [Ct_{mutant (pepc) −} Ct_{mutant (gapdh)}] − [Ct_{wild type (pepc) –} Ct_{wild type (gapdh)}] and the  % expression of mutant strains is expressed relative to that of wild type, which was taken as 100 %