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. 2013 Dec 10;36(4):843–853. doi: 10.1007/s10529-013-1426-9

Fig. 1.

Fig. 1

PCR analysis of Hyoscyamus niger roots transformed by Agrobacterium rhizogenes LBA1334. M. Marker GeneRuler 100 bp DNA ladder; PCR reactions were performed using as a target DNA isolated from: 1 A. rhizogenes LBA1334 + primer rolB as a positive control, 2 A. rhizogenes LBA1334 + primer rolC as a positive control, 3 A. rhizogenes LBA1334 + primer virG as a positive control, 4 Hairy roots of H. niger + primer rolB, 5 Hairy roots of H. niger + primer rolC, 6 Hairy roots of H. niger + primer virG, 7 Non - transformed roots of H. niger + primer rolB as a negative control, 8 Non - transformed roots of H. niger + primer rolC as a negative control, 9 Non-transformed roots of H. niger + primer virG as a negative control. Arrows show amplified fragments of rolB (423 bp; lanes 1, 4, 7), rolC (626 bp; lanes 2, 5, 8) and virG (273 bp; lanes 3, 6, 9) genes