Table 1.

Alkaloid productivity in H. niger hairy roots grown in: SF, BCB and bubble-column/spray HB

Experimental conditions	Day of the culture	Productivity (mg/l day)
		Scopolamine	Hyoscyamine	Anisodamine	Cuscohygrine	Tropane alkaloids	Total alkaloids
SF	30f	1.28 ± 0.07	0.21 ± 0.03	0.04 ± 0.002	4 ± 0.36	1.53 ± 0.08	5.53 ± 0.94
BCB	50f	1 ± 0.09	0.17 ± 0.007	0.02 ± 0.002	4.99 ± 0.25	1.19 ± 0.07	6.18 ± 0.94
HB	30f	0.98 ± 0.13	0.31 ± 0.03	0.07 ± 0.006	5.36 ± 0.12	1.36 ± 0.11	6.72 ± 1.14
HBa	26	0.62 ± 0.06	0.15 ± 0.005	0.05 ± 0.006	2.65 ± 0.1	0.82 ± 0.55	3.47 ± 0.49
HBb	50	0.38 ± 0.04	0.1 ± 0.01	0.12 ± 0.01	1.89 ± 0.18	0.60 ± 0.05	2.49 ± 0.57
HB + MeJc	26	1.58 ± 0.006	0.37 ± 0.02	0.06 ± 0.003	6.19 ± 0.4	2.01 ± 0.01	8.21 ± 0.38
HB + DMSOd	30	0.47 ± 0.09	0.47 ± 0.02	0.18 ± 0.04	2.03 ± 0.91	1.12 ± 0.07	3.42 ± 0.98
HB + XAD-2e	50	0.76 ± 0.001	0.05 ± 0.004	0.1 ± 0.008	2.42 ± 0.19	0.91 ± 0.01	3.33 ± 0.2

Values represent the mean of 3 for SF and 2 for BCB, HB samples ± SD

^aControl for elicitation experiments in HB

^bControl for in situ extraction experiments in HB

^cHairy roots elicited with 1 mM methyl jasmonate on the 26th day

^dHairy roots permeabilized with 20 % (v/v) dimethyl sulfoxide on the 30th day

^eCulture subjected to in situ extraction with Amberlite XAD-2 resin from 30th to 50th day

^fThe day on which maximum productivity of the respective system was obtained