Table 4.
Proteomic profile of LV-IVS of DCM and ICM failing hearts
| Accession | Reference | pI | MW | t-test (P > 95%) | ISC (SpC) | DIL (SpC) | G-test (P > 95%) | DAVE | DCI | Uniprot | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 881394 | Uridine diphosphoglucose pyrophosphorylase | 8.3 | 55702 | * | 0.00 | 0.59 | −2.00 | −17 | Up-regulated in Dilated | Q16851 | |
| 2627129 | Polyubiquitin | 7.7 | 68492 | * | 0.00 | 1.09 | −2.00 | −59 | P0CG48 | ||
| 7023143 | 2-Oxoglutarate dehydrogenase-like | 6.2 | 114480 | * | 0.00 | 0.81 | −2.00 | −33 | Q9ULD0 | ||
| 10584324 | Xin actin-binding repeat-containing protein 1 | 6.8 | 112075 | * | 0.11 | 0.97 | −1.58 | −46 | Q702N8 | ||
| 4503471 | Elongation factor 1-alpha 1 | 9.4 | 50142 | * | 0.22 | 1.36 | −1.45 | −90 | P68104 | ||
| 4504183 | Glutathione S-transferase P | 5.3 | 23357 | * | 0.46 | 2.04 | −1.26 | −197 | P09211 | ||
| 2952333 | Arg/Abl-interacting protein ArgBP2b | 9.9 | 70661 | * | 2.23 | 4.32 | −0.64 | −682 | O94875 | ||
| 4503057 | Alpha-crystallin B chain | 6.9 | 20160 | 50.74 | 83.98 | * | −0.49 | −223887 | P02511 | ||
| 2058322 | Myosin binding protein C gene | 6.2 | 140748 | 41.55 | 61.38 | * | −0.39 | −102089 | Q14896 | ||
| 115496169 | Myosin-7 | 5.5 | 223097 | 100.36 | 135.90 | * | −0.30 | −419833 | P12883 | ||
| 4501867 | Aconitate hydratase, mitochondrial | 7.3 | 85407 | * | 56.36 | 73.39 | −0.26 | −110483 | Q99798 | ||
| 297024 | Cardiac alpha-myosin heavy chain | 5.5 | 223705 | 257.64 | 334.43 | * | −0.26 | −2273187 | P13533 | ||
| 4502027 | Serum albumin | 5.9 | 69367 | 248.82 | 197.58 | * | 0.23 | 1143714 | Up-regulated in Ischemic | P02768 | |
| 14016 | Cytochrome c oxidase II subunit | 4.5 | 20995 | * | 15.27 | 11.65 | 0.27 | 4878 | P00403 | ||
| 509291 | H+-ATP synthase subunit b | 9.7 | 28895 | * | 18.83 | 14.33 | 0.27 | 7455 | P24539 | ||
| 4503607 | Electron transfer flavoprotein subunit alpha | 8.5 | 35061 | * | 28.55 | 19.81 | 0.36 | 21126 | P13804 | ||
| 4557032 | l-lactate dehydrogenase B chain | 5.7 | 36639 | * | 56.29 | 38.42 | 0.38 | 84607 | P07195 | ||
| 190885499 | Cytochrome c oxidase subunit 5A | 6.4 | 16744 | * | 31.38 | 21.32 | 0.38 | 26506 | P20674 | ||
| 6005854 | Prohibitin-2 | 10.2 | 33297 | * | 8.77 | 5.53 | 0.45 | 2316 | Q99623 | ||
| 46593007 | Cytochrome b–c1 complex | 5.9 | 52627 | * | 9.58 | 5.22 | 0.59 | 3227 | P31930 | ||
| 4501885 | Actin, cytoplasmic 1 | 5.2 | 41738 | * | 9.41 | 4.99 | 0.61 | 3183 | P60709 | ||
| 4503143 | Cathepsin D | 6.1 | 44553 | * | 12.07 | 5.92 | 0.68 | 5532 | P07339 | ||
| 6681764 | NADH dehydrogenase [ubiquinone] 1 alpha | 10.2 | 42491 | * | 6.64 | 3.20 | 0.70 | 1689 | Q16795 | ||
| 50363217 | Alpha-1-antitrypsin | 5.3 | 46737 | 19.94 | 8.55 | * | 0.80 | 16216 | P01009 | ||
| 4758774 | NADH dehydrogenase [ubiquinone] 1 beta | 8.6 | 20759 | * | 1.49 | 0.31 | 1.30 | 106 | O96000 | ||
| 2414492 | Immunoglobulin heavy chain, constant region | 6.0 | 25732 | * | 3.69 | 0.74 | 1.33 | 654 | |||
| 167857790 | Alpha-1-acid glycoprotein 1 | 4.9 | 23521 | * | 4.07 | 0.75 | 1.38 | 799 | P02768 | ||
| 7705501 | Transmembrane protein 14C | 10.2 | 11566 | * | 0.72 | 0.12 | 1.45 | 25 | Q9P0S9 | ||
| 4758786 | NADH dehydrogenase [ubiquinone] iron-sulfur | 7.3 | 52527 | * | 0.49 | 0.08 | 1.45 | 12 | O75306 | ||
| 40789020 | KIAA1009 | 5.4 | 155046 | * | 1.63 | 0.23 | 1.50 | 130 | Q5TB80 | ||
| 1620396 | Haptoglobin | 6.4 | 39008 | * | 1.89 | 0.08 | 1.84 | 179 | P00739 | ||
| 223029410 | Talin-1 | 5.7 | 269766 | * | 0.56 | 0.00 | 2.00 | 16 | Q9Y490 |
Specific myocardial proteins were identified by comparing the protein lists of DCM-and ICM-IVS against the average protein profile of normal cardiac tissue, by means of MAProMa software (through calculation of Dave/DCI values). The up-regulated proteins in DCM were down-regulated in ICM, and vice versa. pI, isolectric point; MW, molecular weight.
*
P-value statistically significant.