Figure 2.

Regulation of angiotensinogen (AGT) and endothelin-1 (EDN1) mRNA expression during diabetes in the absence and presence of miR-133a overexpression. Hearts of the animals from the four groups (ntg, ntg/D, miR-133a and miR-133a/D) were isolated and mRNA expression for AGT (A) and EDN1 (B) for these groups was analysed by real-time PCR. Diabetes-induced up-regulation of these transcripts was prevented in the miR-133a animals. Expression data were normalized to that of the reference gene, 18S. n = 6–8/group; values are mean ± SEM. *Significantly different from ntg group, #significantly different from ntg/D group, P < 0.05.