Figure 4.

Characterization of urethral construct collected at 3 weeks after pre-incubation inside the subcutaneous ventral penile cavity. (A) The construct. Green arrow indicates the body of the construct, the blue arrow indicates the foreskin (right side), the yellow arrow indicates the urethra and the black arrow indicates the foreskin (left side). (B) Reticular structures distributed with fibrous bundles (black arrow), small vessels (yellow arrow) and muscle fibres (green arrow) found in the construct under the microscope (bar = 100 μm). (C) Magnification of the structures from B (D) Vessel structure in the construct under the microscope (bar = 100 μm). (E) Muscle cell nature of the construct. Paraffin-embodied specimen of construct was prepared. Continuous sections were then prepared and stained by haematoxylin–eosin staining or against muscle markers, MyoD and Desmin (fluorescent images, bar = 100 μm). (F) Co-expression of desmin and human-specific nuclei antigen in the human umbilical cord mesenchymal stem cells-and minced muscle-derived regenerate under a laser scanning confocal microscope. MyoD indicates myogenic differentiation antigen.