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. 2014 Jan 13;18(3):444–454. doi: 10.1111/jcmm.12193

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© 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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A culture model of proliferating adult colonic circular smooth muscle cells (CSMC) shows the mitogenic role of platelet-derived growth factor (PDGF)-BB. (A–C) Phase contrast images showing typical appearance of CSMC at passage 2 in vitro after plating (A), during growth (B) and at confluence (C). Scale bars: A, 100 μm; B and C, 200 μm. (D) Typical experimental outcome of CSMC growth after 48 hrs in either medium alone (DMEM) or DMEM + FCS using the Wst-8 assay. OD450 nm is proportional to cell number, shown here for triplicate wells per condition. (E) Average growth response of CSMC relative to serum-free control in either 5% FCS or PDGF-BB. All values significantly greater than control (P < 0.05). Cell number is expressed as the ratio of WST-8 reaction product in treated versus control wells, for FCS (n = 8) or PDGF-BB (n = 4), with triplicate wells per experiment. *P < 0.05 versus control; **P > 0.05.