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. 2014 Jan 13;18(3):444–454. doi: 10.1111/jcmm.12193

Figure 2.

Figure 2

Conditioned medium (CM) from the inflamed intestine does not promote growth of circular smooth muscle cells (CSMC) directly, but is mitogenic via platelet-derived growth factor (PDGF)-BB. (A) Representative image of CSMC culture stained for expression of the proliferation marker PCNA and counter-stained for nuclei using H333252, showing positive nuclei (magenta; arrows) among predominantly negative nuclei (blue). Scale bar, 40 μm. (B) Quantification of increased expression of PCNA in CSMC exposed to PDGF-BB (10 ng/ml) or PDGF-BB combined with CM from control or inflamed colon. CM from inflamed but not control colon increased PCNA expression above the effect of PDGF-BB alone (*P < 0.05 versus control medium (DMEM); **P < 0.05 versus control CM + PDGF-BB). (C) CSMC proliferation assay showing that CM from control rat colon or from colon at Day 2 of TNBS-induced colitis did not increase CSMC proliferation (left; P > 0.05 versus untreated control). However, CM from the inflamed colon significantly increased growth when combined with PDGF-BB (right; *P < 0.05).