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. 2014 Jan 13;18(3):444–454. doi: 10.1111/jcmm.12193

Figure 4.

Figure 4

Pro-inflammatory cytokines cause increased expression of platelet-derived growth factor (PDGF)-Rβ without promoting receptor activation. (A) Cytokine treatment did not affect the phosphorylation of PDGF-Rβ by PDGF-BB. Representative images of phosphorylation assay showing similar outcome after treatment with PDGF-BB of control or cytokine-treated circular smooth muscle cells (CSMC). Cells were treated with cytokine (50 ng/ml) for 4 hrs before addition of PDGF-BB (50 ng/ml) for 15 min. C, untreated control. (B) Outcome of qPCR showing significant increase in mRNA for PDGF-Rβ in CSMC at 17 hrs after exposure to interleukin (IL)-1β or tumour necrosis factor (TNF)-α versus control CSMC (*P < 0.05 versus untreated control). (C) Representative image of immunoblot showing increased expression of PDGF-Rβ in CSMC treated with IL-1β or TNF-α (50 ng/ml) for 24 hrs. Loading was normalized through a constant cell number (5000 CSMC) per lane. (D) Quantification of western blotting of CSMC showing increased expression of PDGF-Rβ with IL-1β or TNF-α treatment (*P < 0.05; n = 3), while no increase occurred with transforming growth factor (TGF)-β (P > 0.05).