Figure 1.

Decrease in cyp26a1 levels at uterine implantation sites following pCR3.1-cyp26a1 immunization during mice peri-implantation. (A) cyp26a1 levels were significantly reduced at uterine implantation sites based on Western blotting, especially on D5 of mice pregnancy. β-actin was used as a loading control. The bars represent the SD of the mean of the relative value in grey (cyp26a1/β-actin). A paired t-test was used to assess the significance of differences. Pairwise comparisons between each treatment group, *P < 0.05, **P < 0.01. At least, three independent experiments were repeated at each time-point. A total of 36 samples from pregnant mice were assessed. (B) Cyp26a1 levels were obviously reduced at implantation sites of D5 pregnancy based on immunohistochemistry; bars = 25 μm. Three independent experiments were repeated for this time-point. A total of nine samples from pregnant mice were assessed. E, embryo; LE, Luminal epithelium.