Figure 2.

Th17 cell increased in the peripheral blood and the spleen following pCR3.1-cyp26a1 immunization during mice peri-implantation, especially on D5 of mice pregnancy. (A) Flow cytometry scatterplot of Th17 cells. Th17 cells are CD4⁺ RORγt⁺. (B) The Th17 cell proportion increased significantly in the peripheral blood and the spleen under the influence of recombinant plasmid, especially on D5 of pregnancy. The CD4⁺ RORγt⁺ Th17 cell ratio was evaluated by flow cytometry analysis. The data are expressed as the% of CD4⁺ RORγt⁺ double positive cells. The bars represent the SD of the mean. A paired t-test was used to assess the significance of differences. Pairwise comparisons between each treatment group, *P < 0.05. At least, three independent experiments were repeated for each time-point. A total of 49 samples from pregnant mice were assessed.