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. 2013 Dec 10;18(3):455–467. doi: 10.1111/jcmm.12196

Figure 3.

Figure 3

Decrease in Th17 cells at uterine implantation sites following pCR3.1-cyp26a1 immunization during mice peri-implantation. (A) Th17 levels were significantly reduced at uterine implantation sites based on Western blotting, especially on D5 of mice pregnancy. RORγt is the specific marker of Th17 cell, IL-17 is the specific cytokines secreted by Th17 cells. β-actin was used as a loading control. The bars represent the SD of the mean of the relative value in grey (RORγt/β-actin or IL-17/β-actin). A paired t-test was used to assess the significance of differences. Pairwise comparisons between each treatment group, *P < 0.05, **P < 0.01. At least, three independent experiments were repeated for each time-point. A total of 36 samples from pregnant mice were assessed. (B) Th17 levels were obviously reduced at implantation sites of D5 pregnancy based on RORγt and IL-17 immunohistochemistry stain; bars = 200 and 25 μm. At least, three independent experiments were repeated for this time-point. A total of nine samples from pregnant mice were assessed. E, embryo; S, uterine stroma; LE, Luminal epithelium; GE, Glandular epithelium.