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. 2013 Dec 10;18(3):455–467. doi: 10.1111/jcmm.12196

Figure 5.

Figure 5

Further certainty, the regulation of cyp26a1 on Th17 cells at uterine implantation sites by tail vein injection of cyp26a1 lentiviral interference segment (No. 306) during mice peri-implantation. (A) Decrease in cyp26a1 levels at uterine implantation sites of D5 following tail vein injection of cyp26a1 lentiviral interference segment (306) on D3 of pregnancy. Cyp26a1 levels were reduced at uterine implantation sites based on Western blotting and immunohistochemistry; bars = 25 μm. The data were derived from two separate samples from pregnant mice. At least, three independent experiments were repeated for this time-point. A total of six samples from pregnant mice were assessed. (B) Th17 cell increased in the peripheral blood, but the spleen following tail vein injection of cyp26a1 lentiviral interference segment (No. 306). Flow cytometry histogram of Th17 cells is on the left side. Th17 cells are CD4+ RORγt+. Th17 cell ratio was evaluated by flow cytometry analysis. The data are expressed as the% of CD4+ RORγt+ double positive cells. The bars represent the SD of the mean. A paired t-test was used to assess the significance of differences. Pairwise comparisons between each treatment group, *P < 0.05. Three independent experiments were repeated. A total of nine samples from pregnant mice were assessed. (C) Th17 levels were significantly reduced at uterine implantation sites based on Western blotting of D5 pregnancy. β-actin was used as a loading control. The bars represent the SD of the mean of the relative value in grey (RORγt/β-actin or IL-17/β-actin). A paired t-test was used to assess the significance of differences; *P < 0.05, **P < 0.01. The data were derived from two separate samples from pregnant mice. Three independent experiments were repeated for this time-point. A total of six samples from pregnant mice were assessed. (D) Th17 levels were obviously reduced at implantation sites of D5 pregnancy based on immunohistochemistry of RORγt and IL-17; bars = 200 and 25 μm. The data were derived from four separate samples from pregnant mice. At least, three independent experiments were repeated for this time-point. A total of six samples from pregnant mice were assessed. E, embryo; S, uterine stroma; LE, Luminal epithelium; GE, Glandular epithelium.