Figure 6.

(A) In untreated keratinocytes from perilesional vitiligo skin and in keratinocytes from perilesional vitiligo skin after 48 hrs treatment of SIRT1 siRNA 100 nM, SIRT1 protein expression was determined by Western blot analysis. (B) To evaluate the possible involvement of SIRT1 in oxidative-mediated damage of vitiligo keratinocytes, untreated and SIRT1 RNAi-treated keratinocytes from healthy vitiligo skin were challenged for 3 hrs with 500 μM H₂O₂. Reactive oxygen species production, lipid peroxidation, caspase-3 activity and mitochondrial membrane polarization were measured by flow cytometry. These data confirm the key role of SIRT1 in vitiligo. The reported values (means ± SD) are representative of three independent experiments, each performed in triplicate. *Significant difference (P ≤ 0.05) versus untreated perilesional keratinocytes.