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. 2014 Jan 22;18(3):542–553. doi: 10.1111/jcmm.12208

Figure 6.

Figure 6

The effect of siRNA-APJ on the proliferation and activation of PI3K/Akt/mTOR signals in pulmonary arterial smooth muscle cells (PASMCs) under hypoxia. (A) Western blot analysis of APJ receptor protein expression in PASMCs transfected with siRNA-APJ and scramble vectors as described above for 24 hrs. (B) Densitometry was applied to quantify the protein density. Data were presented as a mean ± SD from three independent experiments. #P < 0.01 versus scramble group. (C) PASMCs treated with siRNA-APJ and scramble siRNA vectors for 24 hrs, cell proliferation was measured by 5-bromo-2′-deoxyuridine (BrdU) assay. *P < 0.05 versus hypoxia group. #P < 0.05 versus apelin-treated hypoxia group. n = 5. (D) Phosphorylation of PI3K/Akt/mTOR protein in PASMCs treated with siRNA-APJ and apelin in hypoxia condition. (E) Densitometry was applied to quantify the protein density; data were presented as a mean ± SD from three independent experiments. *P < 0.05 versus apelin-treated hypoxia group.