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. 2014 Jan 22;18(3):542–553. doi: 10.1111/jcmm.12208

Figure 7.

Figure 7

Transfection of siRNA-APJ blocks the inhibitory effect of apelin on autophagy in pulmonary arterial smooth muscle cells (PASMCs) under hypoxia. PASMCs treated with apelin and transfected with siRNA-APJ in hypoxia conditions. (A) Representative images of PASMCs were stained with DAPI (blue) and antibodies against LC3 (green). Images are at 1000×. Microphotographs were shown as representative results from three independent experiments. (B) The corresponding linear diagram of LC3 staining. (C) The protein levels of ATG4B and LC3 were detected with immunoblotting. (D) The ratio of normalized LC3-II to LC3-I. Data were presented as a mean ± SD from three independent experiments. *P < 0.05 versus control group, #P < 0.05 versus hypoxia group, $P < 0.05 versus apelin-treated hypoxia group. (E) The ratio of normalized ATG4B protein. Data were presented as a mean ± SD from three independent experiments. *P < 0.05 versus control group.